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  • The Italian Collection of Antarctic Bacteria (CIBAN) was established in 1989 at the Department of Chemical, Biological, Pharmaceutical and Environmental Sciences of the University of Messina. CIBAN is one of the few collections in the world dedicated to Antarctic bacteria and, since its inception, it has grown to include 515 cold-adapted strains belonging to 69 Genera. Bacteria were extracted from seawater, sediment, and benthic organisms, mainly from the Terra Nova Bay marine area in East Antarctica. CIBAN also hosts bacterial isolates from the continent. Quality control: Strains are routinely streaked on agar plates every six months to control purity and viability. Method steps: All cultures are maintained in duplicate on agar slants at 4 °C. Antarctic strains are also preserved by freezing cell suspensions at – 80 °C in liquid broth to which 20% glycerol is added.